Polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III binding to ssDNA
نویسندگان
چکیده
منابع مشابه
Polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III α binding to ssDNA
Replication by Escherichia coli DNA polymerase III is disrupted on encountering DNA damage. Consequently, specialized Y-family DNA polymerases are used to bypass DNA damage. The protein UmuD is extensively involved in modulating cellular responses to DNA damage and may play a role in DNA polymerase exchange for damage tolerance. In the absence of DNA, UmuD interacts with the α subunit of DNA po...
متن کاملDNA Polymerase III of Escherichia coli
DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...
متن کاملDNA polymerase III holoenzyme of Escherichia coli.
DNA polymerase III holoenzyme has been purified from Escherichia coli HMS-83, using, as an assay, the conversion of coliphage G4 single-stranded DNA to the duplex replicative form. The holoenzyme consists of at least four different subunits: a, /I, y, and 6 of 140,000, 40,000, 52,000, and 32,000 daltons, respectively. The (Y subunit is DNA polymerase III, the dnaE gene product. The holoenzyme h...
متن کاملDNA Polymerase III of Escherichia coli
DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...
متن کاملDNA Polymerase III of Escherichia coli
DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83. The enzyme contains three subunits: (Y, E, and 0 of 140,000, 25,000, and 10,000 daltons, respectively. The cy subunit has been previously shown to be a component of both DNA polymerase III and the more complex DNA polymerase III holoenzyme (Livingston,...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2013
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkt648